Sabrina Moyo sin midtveisevaluering finner sted onsdag 15.05 klokken 13:00 i seminarrommet i 8 etasje, Laboratoriebygget. Alle som ønsker å delta er velkommen.
Project title: Diarrhoeal diseases in children of Dar es Salaam Tanzania with emphasis on viral diarrhoea
Objectives and methods: The project aimed at determining the prevalence of enteric viruses and molecular characterization of other enteric viruses’ i.e. Rotavirus, Noroviorus and adenovirus. We also looked at Seasonal variations in distribution of enteric viruses the impact of HIV and other clinical characteristics in the occurrence of enteric viruses between children with diarrhoea and age matched controls. Stool specimen was collected from under two years, 703 children with diarrhoea and 563 children without diarrhoea. Demographic, clinical information and HIV status was also recorded. ELISA was performed for detection of rotavirus and adenovirus. Extraction of total nucleic acid i.e. RNA and DNA from stool samples was done. Norovirus (GI and GII) detection was done using one step real time PCR. PCR and sequencing was done for typing of samples tested positive for Rotavirus, adenoviruses and Norovrius.
Results
Subproject-1 (Title): Changing pattern of rotavirus strains in Dar es Salaam Tanzania during pre-vaccination 2010-2011: detection of serotype G8 and G12 (status: in manuscript form)
• The prevalence of rotavirus infection was significantly higher in cases than in controls (32.5% vs.7.7%), OR= 5.8. CI 95%CI (4.045-8.192).
• The most commonly detected G type strains were G1 126(68.9%), followed by G8 (16.4), G12 (12.0%), G4 (2.2%) and only one strain of G9 (0.5%).
• The commonest P type circulating was P [8] 127(69.4) followed by P [6] (17.5%) and P [4] 13.1%.
• The commonest G/P combination was G1P [8] accounting for 126 strains, followed by G12 P [6] 22 strains and G8 P [4] 22 strains.
Subproject-2(title): Molecular epidemiology and characterisation of norovirus in Dar es Salaam Tanzania. (Status: Writing manuscript)
• Norovirus was detected in 182 samples from 181 children.
• The prevalence of norovirus was significantly higher in cases than in controls, 18.3% and 9.2% respectively, P<0.02, RR=2.2 and CI (1.56-3.09).
• All cases were GII except one child who had both GII and GI. Controls were infected with both GI 23.1% (12/52) and GII 76.9% (40/52).
Subproject-3 (Title) Detection of adenovirus in children hospitalised with diarrhoea and children without diarrhoea, in Dar es Salaam, Tanzania (status: writing manuscript)
• In total HAdv was detected in 37 out of 1235 children.
• The prevalence was not significantly higher in cases than in controls (3.5 vs. 2.4 %) (P>0.05).
• Serotype 40, and 41 accounted for 50% (12/24) of adenovirus positive samples; followed by serotype 31, 7 and 2 all had similar frequency of 12.5 % (3/24). Other serotypes were 18(8.3%) and 1 (4.2%).
• No clear pattern of seasonality was found.